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mouse monoclonal  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology mouse monoclonal
    Mouse Monoclonal, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 65 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+monoclonal+anti+omp/OMP+Antibody/bio_rxiv__64898__2025__12__30__696934-86-46-52
    Average 94 stars, based on 65 article reviews
    mouse monoclonal - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Staining:

    Article Title: The Aquaporin-4 Expression and Localization in the Olfactory Epithelium Modulate the Odorant-Evoked Responses and Olfactory-Driven Behavior.
    Article Snippet: .. Slices stained with CYT8 and S100B were incubated with 0.5% (w/v) SDS in PBS Ca2+- Mg2+, followed by blocking solution 5% (v/v) donkey serum, 2% (w/v) BSA, 0.1% (w/v) Triton X100 in PBS Ca2+- Mg2+ for 60 min. Then, the slices were incubated overnight at 4°C with rabbit polyclonal anti- AQP4 (GenScript Biotech, Piscataway, NJ, USA) diluted 1:2000 in the blocking buffer, rabbit polyclonal anti- AQP4ex generated against the peptide DSTEGRRDSLDLASC within the AQP4 C- terminus (1:1000; GenScript Biotech), mouse monoclonal anti- OMP (1:300; Santa Cruz Biotechnology, TX, USA), rat monoclonal anti- Sox2 (1:300; eBioscience, Invitrogen, San Diego, USA), rabbit polyclonal anti- Kir 4.1 (1:100, Alomone Labs, Jerusalem, Israel), rabbit polyclonal anti- Ki67 (1:200; Abcam, Cambridge, UK), rat polyclonal anti- CYT8–TROMA 1 (1:100; DSHB), rabbit polyclonal S100B (1:300, Proteintech, San Diego, California). .. Slices were incubated with AlexaFluor 488 goat anti- rabbit, AlexaFluor 594 donkey anti- rabbit, and AlexaFluor 594 goat anti- mouse at 1:1000, and AlexaFluor 488 donkey anti- rat (1:500) in 0.2% (w/v) Tween 20, PBS Ca2+- Mg2+ (all from Life Technologies, Thermo Fisher Scientific, Carlsbad, California, USA) for 45 min at room temperature.

    Incubation:

    Article Title: The Aquaporin-4 Expression and Localization in the Olfactory Epithelium Modulate the Odorant-Evoked Responses and Olfactory-Driven Behavior.
    Article Snippet: .. Slices stained with CYT8 and S100B were incubated with 0.5% (w/v) SDS in PBS Ca2+- Mg2+, followed by blocking solution 5% (v/v) donkey serum, 2% (w/v) BSA, 0.1% (w/v) Triton X100 in PBS Ca2+- Mg2+ for 60 min. Then, the slices were incubated overnight at 4°C with rabbit polyclonal anti- AQP4 (GenScript Biotech, Piscataway, NJ, USA) diluted 1:2000 in the blocking buffer, rabbit polyclonal anti- AQP4ex generated against the peptide DSTEGRRDSLDLASC within the AQP4 C- terminus (1:1000; GenScript Biotech), mouse monoclonal anti- OMP (1:300; Santa Cruz Biotechnology, TX, USA), rat monoclonal anti- Sox2 (1:300; eBioscience, Invitrogen, San Diego, USA), rabbit polyclonal anti- Kir 4.1 (1:100, Alomone Labs, Jerusalem, Israel), rabbit polyclonal anti- Ki67 (1:200; Abcam, Cambridge, UK), rat polyclonal anti- CYT8–TROMA 1 (1:100; DSHB), rabbit polyclonal S100B (1:300, Proteintech, San Diego, California). .. Slices were incubated with AlexaFluor 488 goat anti- rabbit, AlexaFluor 594 donkey anti- rabbit, and AlexaFluor 594 goat anti- mouse at 1:1000, and AlexaFluor 488 donkey anti- rat (1:500) in 0.2% (w/v) Tween 20, PBS Ca2+- Mg2+ (all from Life Technologies, Thermo Fisher Scientific, Carlsbad, California, USA) for 45 min at room temperature.

    Article Title: The Aquaporin-4 Expression and Localization in the Olfactory Epithelium Modulate the Odorant-Evoked Responses and Olfactory-Driven Behavior.
    Article Snippet: .. Hence, membranes were incubated overnight or for 1.5 h at 4°C in blocking buffer with the following primary antibodies: rabbit polyclonal anti- AQP4 (1:4000, GenScript Biotech), rabbit polyclonal anti- AQP4ex (1:2000; GenScript Biotech), mouse monoclonal anti- OMP (1:1000; Santa Cruz Biotechnology), rabbit polyclonal anti- Kir 4.1 (1:400, Alomone Labs), rabbit polyclonal anti- S100B (1:400, Proteintech). .. Then, they were washed in blocking buffer and incubated with the following peroxidase- conjugated secondary antibodies at room temperature for 45 min: goat anti- rabbit IgG HRP (1:3000; Bio- Rad, California, USA) and goat antimouse IgG HRP (1:3000; Bio- Rad).

    Article Title: The Aquaporin-4 expression and localization in the olfactory epithelium modulate the odorant-evoked responses and olfactory driven behavior
    Article Snippet: Briefly, proteins were separated on 13% acrylamide/bis-acrylamide gel for AQP4 (20 μg), AQP4ex (20 μg) and Kir 4.1 (20 μg) and 15% acrylamide/bis-acrylamide gel for OMP (35 μg), and transferred to polyvinylidenedifluoride (PVDF) membranes (Millipore, Burlington, Massachusetts, USA) for immunoblot analysis. .. Membranes were incubated overnight at 4°C with the following primary antibodies: rabbit polyclonal anti-AQP4 (1:4000, GenScript Biotech, Piscataway, NJ, USA), rabbit polyclonal anti-AQP4ex (1:2000; GenScript Biotech, Piscataway, NJ, USA), mouse monoclonal anti-OMP (1:1000; Santa Cruz, Biotechnology, TX, USA), rabbit polyclonal anti-Kir 4.1 (1:400, Alomone Labs, Jerusalem, Israel). .. Then washed and incubated with the following peroxidase-conjugated secondary antibodies at room temperature for 45 min: goat anti-rabbit IgG HRP (1:3000; Bio-Rad, California, USA) and goat anti-mouse IgG HRP (1:3000; Bio-Rad, California, USA).

    Article Title: The Aquaporin-4 expression and localization in the olfactory epithelium modulate the odorant-evoked responses and olfactory driven behavior
    Article Snippet: Coronal sections 14-15 μm thick were cut with a cryostat (CM 1900, Leica) at -20°C and stored on poly-L-lysine glass slides at -80°C. .. After rehydration with PBS Ca 2+ -Mg 2+ , sections were incubated with SDS 0.5% (w/v) in PBS Ca 2+ - Mg 2+ for antigen retrieval, followed by blocking solution 5% (v/v) normal goat serum, 4% (w/v) BSA, 5% (w/v) non-fat dry milk, 0.3% (w/v) Triton X-100 in PBS Ca 2+ -Mg 2+ , for 30 min. Then the slices were incubated overnight at 4°C with rabbit polyclonal anti-AQP4 (GenScript Biotech, Piscataway, NJ, USA) diluted 1:2000 in the blocking buffer, rabbit polyclonal anti-AQP4ex generated against the peptide DSTEGRRDSLDLASC within the AQP4 C-terminus (1:1000; GenScript Biotech, Piscataway, NJ, USA), mouse monoclonal anti- OMP (1:300; Santa Cruz Biotechnology, TX, USA), rat monoclonal anti-Sox2 (1:300; eBioscience, Invitrogen, San Diego, USA), rabbit polyclonal anti-Kir 4.1 (1:200, Alomone Labs, Jerusalem, Israel), rabbit polyclonal anti-Ki67 (1:200; Abcam, Cambridge, UK). .. AlexaFluor 488 goat anti-rabbit, AlexaFluor 594 donkey anti-rabbit, and AlexaFluor 594 goat anti-mouse were used at 1:1000, and AlexaFluor 488 donkey anti-rat (1:500) in 0.2% (w/v) Tween 20, PBS Ca 2+ -Mg 2+ (all from Life Technologies, Thermo Fisher Scientific, Carlsbad, California, USA) for 45 min at room temperature.

    Blocking Assay:

    Article Title: The Aquaporin-4 Expression and Localization in the Olfactory Epithelium Modulate the Odorant-Evoked Responses and Olfactory-Driven Behavior.
    Article Snippet: .. Slices stained with CYT8 and S100B were incubated with 0.5% (w/v) SDS in PBS Ca2+- Mg2+, followed by blocking solution 5% (v/v) donkey serum, 2% (w/v) BSA, 0.1% (w/v) Triton X100 in PBS Ca2+- Mg2+ for 60 min. Then, the slices were incubated overnight at 4°C with rabbit polyclonal anti- AQP4 (GenScript Biotech, Piscataway, NJ, USA) diluted 1:2000 in the blocking buffer, rabbit polyclonal anti- AQP4ex generated against the peptide DSTEGRRDSLDLASC within the AQP4 C- terminus (1:1000; GenScript Biotech), mouse monoclonal anti- OMP (1:300; Santa Cruz Biotechnology, TX, USA), rat monoclonal anti- Sox2 (1:300; eBioscience, Invitrogen, San Diego, USA), rabbit polyclonal anti- Kir 4.1 (1:100, Alomone Labs, Jerusalem, Israel), rabbit polyclonal anti- Ki67 (1:200; Abcam, Cambridge, UK), rat polyclonal anti- CYT8–TROMA 1 (1:100; DSHB), rabbit polyclonal S100B (1:300, Proteintech, San Diego, California). .. Slices were incubated with AlexaFluor 488 goat anti- rabbit, AlexaFluor 594 donkey anti- rabbit, and AlexaFluor 594 goat anti- mouse at 1:1000, and AlexaFluor 488 donkey anti- rat (1:500) in 0.2% (w/v) Tween 20, PBS Ca2+- Mg2+ (all from Life Technologies, Thermo Fisher Scientific, Carlsbad, California, USA) for 45 min at room temperature.

    Article Title: The Aquaporin-4 Expression and Localization in the Olfactory Epithelium Modulate the Odorant-Evoked Responses and Olfactory-Driven Behavior.
    Article Snippet: .. Hence, membranes were incubated overnight or for 1.5 h at 4°C in blocking buffer with the following primary antibodies: rabbit polyclonal anti- AQP4 (1:4000, GenScript Biotech), rabbit polyclonal anti- AQP4ex (1:2000; GenScript Biotech), mouse monoclonal anti- OMP (1:1000; Santa Cruz Biotechnology), rabbit polyclonal anti- Kir 4.1 (1:400, Alomone Labs), rabbit polyclonal anti- S100B (1:400, Proteintech). .. Then, they were washed in blocking buffer and incubated with the following peroxidase- conjugated secondary antibodies at room temperature for 45 min: goat anti- rabbit IgG HRP (1:3000; Bio- Rad, California, USA) and goat antimouse IgG HRP (1:3000; Bio- Rad).

    Article Title: The Aquaporin-4 expression and localization in the olfactory epithelium modulate the odorant-evoked responses and olfactory driven behavior
    Article Snippet: Coronal sections 14-15 μm thick were cut with a cryostat (CM 1900, Leica) at -20°C and stored on poly-L-lysine glass slides at -80°C. .. After rehydration with PBS Ca 2+ -Mg 2+ , sections were incubated with SDS 0.5% (w/v) in PBS Ca 2+ - Mg 2+ for antigen retrieval, followed by blocking solution 5% (v/v) normal goat serum, 4% (w/v) BSA, 5% (w/v) non-fat dry milk, 0.3% (w/v) Triton X-100 in PBS Ca 2+ -Mg 2+ , for 30 min. Then the slices were incubated overnight at 4°C with rabbit polyclonal anti-AQP4 (GenScript Biotech, Piscataway, NJ, USA) diluted 1:2000 in the blocking buffer, rabbit polyclonal anti-AQP4ex generated against the peptide DSTEGRRDSLDLASC within the AQP4 C-terminus (1:1000; GenScript Biotech, Piscataway, NJ, USA), mouse monoclonal anti- OMP (1:300; Santa Cruz Biotechnology, TX, USA), rat monoclonal anti-Sox2 (1:300; eBioscience, Invitrogen, San Diego, USA), rabbit polyclonal anti-Kir 4.1 (1:200, Alomone Labs, Jerusalem, Israel), rabbit polyclonal anti-Ki67 (1:200; Abcam, Cambridge, UK). .. AlexaFluor 488 goat anti-rabbit, AlexaFluor 594 donkey anti-rabbit, and AlexaFluor 594 goat anti-mouse were used at 1:1000, and AlexaFluor 488 donkey anti-rat (1:500) in 0.2% (w/v) Tween 20, PBS Ca 2+ -Mg 2+ (all from Life Technologies, Thermo Fisher Scientific, Carlsbad, California, USA) for 45 min at room temperature.

    Generated:

    Article Title: The Aquaporin-4 Expression and Localization in the Olfactory Epithelium Modulate the Odorant-Evoked Responses and Olfactory-Driven Behavior.
    Article Snippet: .. Slices stained with CYT8 and S100B were incubated with 0.5% (w/v) SDS in PBS Ca2+- Mg2+, followed by blocking solution 5% (v/v) donkey serum, 2% (w/v) BSA, 0.1% (w/v) Triton X100 in PBS Ca2+- Mg2+ for 60 min. Then, the slices were incubated overnight at 4°C with rabbit polyclonal anti- AQP4 (GenScript Biotech, Piscataway, NJ, USA) diluted 1:2000 in the blocking buffer, rabbit polyclonal anti- AQP4ex generated against the peptide DSTEGRRDSLDLASC within the AQP4 C- terminus (1:1000; GenScript Biotech), mouse monoclonal anti- OMP (1:300; Santa Cruz Biotechnology, TX, USA), rat monoclonal anti- Sox2 (1:300; eBioscience, Invitrogen, San Diego, USA), rabbit polyclonal anti- Kir 4.1 (1:100, Alomone Labs, Jerusalem, Israel), rabbit polyclonal anti- Ki67 (1:200; Abcam, Cambridge, UK), rat polyclonal anti- CYT8–TROMA 1 (1:100; DSHB), rabbit polyclonal S100B (1:300, Proteintech, San Diego, California). .. Slices were incubated with AlexaFluor 488 goat anti- rabbit, AlexaFluor 594 donkey anti- rabbit, and AlexaFluor 594 goat anti- mouse at 1:1000, and AlexaFluor 488 donkey anti- rat (1:500) in 0.2% (w/v) Tween 20, PBS Ca2+- Mg2+ (all from Life Technologies, Thermo Fisher Scientific, Carlsbad, California, USA) for 45 min at room temperature.

    Article Title: The Aquaporin-4 expression and localization in the olfactory epithelium modulate the odorant-evoked responses and olfactory driven behavior
    Article Snippet: Coronal sections 14-15 μm thick were cut with a cryostat (CM 1900, Leica) at -20°C and stored on poly-L-lysine glass slides at -80°C. .. After rehydration with PBS Ca 2+ -Mg 2+ , sections were incubated with SDS 0.5% (w/v) in PBS Ca 2+ - Mg 2+ for antigen retrieval, followed by blocking solution 5% (v/v) normal goat serum, 4% (w/v) BSA, 5% (w/v) non-fat dry milk, 0.3% (w/v) Triton X-100 in PBS Ca 2+ -Mg 2+ , for 30 min. Then the slices were incubated overnight at 4°C with rabbit polyclonal anti-AQP4 (GenScript Biotech, Piscataway, NJ, USA) diluted 1:2000 in the blocking buffer, rabbit polyclonal anti-AQP4ex generated against the peptide DSTEGRRDSLDLASC within the AQP4 C-terminus (1:1000; GenScript Biotech, Piscataway, NJ, USA), mouse monoclonal anti- OMP (1:300; Santa Cruz Biotechnology, TX, USA), rat monoclonal anti-Sox2 (1:300; eBioscience, Invitrogen, San Diego, USA), rabbit polyclonal anti-Kir 4.1 (1:200, Alomone Labs, Jerusalem, Israel), rabbit polyclonal anti-Ki67 (1:200; Abcam, Cambridge, UK). .. AlexaFluor 488 goat anti-rabbit, AlexaFluor 594 donkey anti-rabbit, and AlexaFluor 594 goat anti-mouse were used at 1:1000, and AlexaFluor 488 donkey anti-rat (1:500) in 0.2% (w/v) Tween 20, PBS Ca 2+ -Mg 2+ (all from Life Technologies, Thermo Fisher Scientific, Carlsbad, California, USA) for 45 min at room temperature.



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    Image Search Results


    Distribution pattern of β-amyloid. Double immunostaining with TUJ-1 (green) and mAb to β-amyloid (red). Beta-amyloid shows a focal expression (dot-like) in the proximity of the nucleus and at the level of the surface tips of the olfactory neurons [outlined square in (A) , details in (B) , arrows]. Scale bar (A) : 20 μm. Scale bar (B) : 10 μm.

    Journal: Frontiers in Neuroscience

    Article Title: Neurodegeneration-Associated Proteins in Human Olfactory Neurons Collected by Nasal Brushing

    doi: 10.3389/fnins.2020.00145

    Figure Lengend Snippet: Distribution pattern of β-amyloid. Double immunostaining with TUJ-1 (green) and mAb to β-amyloid (red). Beta-amyloid shows a focal expression (dot-like) in the proximity of the nucleus and at the level of the surface tips of the olfactory neurons [outlined square in (A) , details in (B) , arrows]. Scale bar (A) : 20 μm. Scale bar (B) : 10 μm.

    Article Snippet: Anti-OMP , Mouse monoclonal , Olfactory marker protein , sc-365818 (B-6) , Santa Cruz , 1:400.

    Techniques: Double Immunostaining, Expressing

    Distribution pattern of 4R and 3R tau isoform expression. Double immunostaining with TUJ-1 (red) and mAb to 4R tau isoform ( A , B , green) and 3R tau isoform ( C , D , green). (A,B) The 4R tau isoform (green) is mainly localized in the cytosolic apical portion of TUJ-1-positive olfactory neurons, (red) in the proximity of the dendritic knob (arrows). As opposite, TUJ-1-positive cells are negative to the 3R tau isoform (green), which is unevenly distributed as granules in rounded cells, likely in differentiated stem cells, negative for the TUJ-1 antibody ( C , D , *). All scale bars: 10 μm.

    Journal: Frontiers in Neuroscience

    Article Title: Neurodegeneration-Associated Proteins in Human Olfactory Neurons Collected by Nasal Brushing

    doi: 10.3389/fnins.2020.00145

    Figure Lengend Snippet: Distribution pattern of 4R and 3R tau isoform expression. Double immunostaining with TUJ-1 (red) and mAb to 4R tau isoform ( A , B , green) and 3R tau isoform ( C , D , green). (A,B) The 4R tau isoform (green) is mainly localized in the cytosolic apical portion of TUJ-1-positive olfactory neurons, (red) in the proximity of the dendritic knob (arrows). As opposite, TUJ-1-positive cells are negative to the 3R tau isoform (green), which is unevenly distributed as granules in rounded cells, likely in differentiated stem cells, negative for the TUJ-1 antibody ( C , D , *). All scale bars: 10 μm.

    Article Snippet: Anti-OMP , Mouse monoclonal , Olfactory marker protein , sc-365818 (B-6) , Santa Cruz , 1:400.

    Techniques: Expressing, Double Immunostaining