mouse monoclonal (Santa Cruz Biotechnology)
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Mouse Monoclonal, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 65 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+monoclonal+anti+omp/OMP+Antibody/bio_rxiv__64898__2025__12__30__696934-86-46-52
Average 94 stars, based on 65 article reviews
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Staining:Article Title: The Aquaporin-4 Expression and Localization in the Olfactory Epithelium Modulate the Odorant-Evoked Responses and Olfactory-Driven Behavior. Article Snippet: .. Slices stained with CYT8 and S100B were incubated with 0.5% (w/v) SDS in PBS Ca2+- Mg2+, followed by blocking solution 5% (v/v) donkey serum, 2% (w/v) BSA, 0.1% (w/v) Triton X100 in PBS Ca2+- Mg2+ for 60 min. Then, the slices were incubated overnight at 4°C with rabbit polyclonal anti- AQP4 (GenScript Biotech, Piscataway, NJ, USA) diluted 1:2000 in the blocking buffer, rabbit polyclonal anti- AQP4ex generated against the peptide DSTEGRRDSLDLASC within the AQP4 C- terminus (1:1000; GenScript Biotech), Incubation:Article Title: The Aquaporin-4 Expression and Localization in the Olfactory Epithelium Modulate the Odorant-Evoked Responses and Olfactory-Driven Behavior. Article Snippet: .. Slices stained with CYT8 and S100B were incubated with 0.5% (w/v) SDS in PBS Ca2+- Mg2+, followed by blocking solution 5% (v/v) donkey serum, 2% (w/v) BSA, 0.1% (w/v) Triton X100 in PBS Ca2+- Mg2+ for 60 min. Then, the slices were incubated overnight at 4°C with rabbit polyclonal anti- AQP4 (GenScript Biotech, Piscataway, NJ, USA) diluted 1:2000 in the blocking buffer, rabbit polyclonal anti- AQP4ex generated against the peptide DSTEGRRDSLDLASC within the AQP4 C- terminus (1:1000; GenScript Biotech), Article Title: The Aquaporin-4 Expression and Localization in the Olfactory Epithelium Modulate the Odorant-Evoked Responses and Olfactory-Driven Behavior. Article Snippet: .. Hence, membranes were incubated overnight or for 1.5 h at 4°C in blocking buffer with the following primary antibodies: rabbit polyclonal anti- AQP4 (1:4000, GenScript Biotech), rabbit polyclonal anti- AQP4ex (1:2000; GenScript Biotech), Article Title: The Aquaporin-4 expression and localization in the olfactory epithelium modulate the odorant-evoked responses and olfactory driven behavior Article Snippet: Briefly, proteins were separated on 13% acrylamide/bis-acrylamide gel for AQP4 (20 μg), AQP4ex (20 μg) and Kir 4.1 (20 μg) and 15% acrylamide/bis-acrylamide gel for OMP (35 μg), and transferred to polyvinylidenedifluoride (PVDF) membranes (Millipore, Burlington, Massachusetts, USA) for immunoblot analysis. .. Membranes were incubated overnight at 4°C with the following primary antibodies: rabbit polyclonal anti-AQP4 (1:4000, GenScript Biotech, Piscataway, NJ, USA), rabbit polyclonal anti-AQP4ex (1:2000; GenScript Biotech, Piscataway, NJ, USA), Article Title: The Aquaporin-4 expression and localization in the olfactory epithelium modulate the odorant-evoked responses and olfactory driven behavior Article Snippet: Coronal sections 14-15 μm thick were cut with a cryostat (CM 1900, Leica) at -20°C and stored on poly-L-lysine glass slides at -80°C. .. After rehydration with PBS Ca 2+ -Mg 2+ , sections were incubated with SDS 0.5% (w/v) in PBS Ca 2+ - Mg 2+ for antigen retrieval, followed by blocking solution 5% (v/v) normal goat serum, 4% (w/v) BSA, 5% (w/v) non-fat dry milk, 0.3% (w/v) Triton X-100 in PBS Ca 2+ -Mg 2+ , for 30 min. Then the slices were incubated overnight at 4°C with rabbit polyclonal anti-AQP4 (GenScript Biotech, Piscataway, NJ, USA) diluted 1:2000 in the blocking buffer, rabbit polyclonal anti-AQP4ex generated against the peptide DSTEGRRDSLDLASC within the AQP4 C-terminus (1:1000; GenScript Biotech, Piscataway, NJ, USA), Blocking Assay:Article Title: The Aquaporin-4 Expression and Localization in the Olfactory Epithelium Modulate the Odorant-Evoked Responses and Olfactory-Driven Behavior. Article Snippet: .. Slices stained with CYT8 and S100B were incubated with 0.5% (w/v) SDS in PBS Ca2+- Mg2+, followed by blocking solution 5% (v/v) donkey serum, 2% (w/v) BSA, 0.1% (w/v) Triton X100 in PBS Ca2+- Mg2+ for 60 min. Then, the slices were incubated overnight at 4°C with rabbit polyclonal anti- AQP4 (GenScript Biotech, Piscataway, NJ, USA) diluted 1:2000 in the blocking buffer, rabbit polyclonal anti- AQP4ex generated against the peptide DSTEGRRDSLDLASC within the AQP4 C- terminus (1:1000; GenScript Biotech), Article Title: The Aquaporin-4 Expression and Localization in the Olfactory Epithelium Modulate the Odorant-Evoked Responses and Olfactory-Driven Behavior. Article Snippet: .. Hence, membranes were incubated overnight or for 1.5 h at 4°C in blocking buffer with the following primary antibodies: rabbit polyclonal anti- AQP4 (1:4000, GenScript Biotech), rabbit polyclonal anti- AQP4ex (1:2000; GenScript Biotech), Article Title: The Aquaporin-4 expression and localization in the olfactory epithelium modulate the odorant-evoked responses and olfactory driven behavior Article Snippet: Coronal sections 14-15 μm thick were cut with a cryostat (CM 1900, Leica) at -20°C and stored on poly-L-lysine glass slides at -80°C. .. After rehydration with PBS Ca 2+ -Mg 2+ , sections were incubated with SDS 0.5% (w/v) in PBS Ca 2+ - Mg 2+ for antigen retrieval, followed by blocking solution 5% (v/v) normal goat serum, 4% (w/v) BSA, 5% (w/v) non-fat dry milk, 0.3% (w/v) Triton X-100 in PBS Ca 2+ -Mg 2+ , for 30 min. Then the slices were incubated overnight at 4°C with rabbit polyclonal anti-AQP4 (GenScript Biotech, Piscataway, NJ, USA) diluted 1:2000 in the blocking buffer, rabbit polyclonal anti-AQP4ex generated against the peptide DSTEGRRDSLDLASC within the AQP4 C-terminus (1:1000; GenScript Biotech, Piscataway, NJ, USA), Generated:Article Title: The Aquaporin-4 Expression and Localization in the Olfactory Epithelium Modulate the Odorant-Evoked Responses and Olfactory-Driven Behavior. Article Snippet: .. Slices stained with CYT8 and S100B were incubated with 0.5% (w/v) SDS in PBS Ca2+- Mg2+, followed by blocking solution 5% (v/v) donkey serum, 2% (w/v) BSA, 0.1% (w/v) Triton X100 in PBS Ca2+- Mg2+ for 60 min. Then, the slices were incubated overnight at 4°C with rabbit polyclonal anti- AQP4 (GenScript Biotech, Piscataway, NJ, USA) diluted 1:2000 in the blocking buffer, rabbit polyclonal anti- AQP4ex generated against the peptide DSTEGRRDSLDLASC within the AQP4 C- terminus (1:1000; GenScript Biotech), Article Title: The Aquaporin-4 expression and localization in the olfactory epithelium modulate the odorant-evoked responses and olfactory driven behavior Article Snippet: Coronal sections 14-15 μm thick were cut with a cryostat (CM 1900, Leica) at -20°C and stored on poly-L-lysine glass slides at -80°C. .. After rehydration with PBS Ca 2+ -Mg 2+ , sections were incubated with SDS 0.5% (w/v) in PBS Ca 2+ - Mg 2+ for antigen retrieval, followed by blocking solution 5% (v/v) normal goat serum, 4% (w/v) BSA, 5% (w/v) non-fat dry milk, 0.3% (w/v) Triton X-100 in PBS Ca 2+ -Mg 2+ , for 30 min. Then the slices were incubated overnight at 4°C with rabbit polyclonal anti-AQP4 (GenScript Biotech, Piscataway, NJ, USA) diluted 1:2000 in the blocking buffer, rabbit polyclonal anti-AQP4ex generated against the peptide DSTEGRRDSLDLASC within the AQP4 C-terminus (1:1000; GenScript Biotech, Piscataway, NJ, USA), |
![Distribution pattern of β-amyloid. Double immunostaining with TUJ-1 (green) and <t>mAb</t> to β-amyloid (red). Beta-amyloid shows a focal expression (dot-like) in the proximity of the nucleus and at the level of the surface tips of the olfactory neurons [outlined square in (A) , details in (B) , arrows]. Scale bar (A) : 20 μm. Scale bar (B) : 10 μm.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_6258/pmc07066258/pmc07066258__fnins-14-00145-g005.jpg)